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    Home > Biochemistry News > Biotechnology News > How to determine the gene's inclusion and exon sequence and gene structure.

    How to determine the gene's inclusion and exon sequence and gene structure.

    • Last Update: 2020-10-23
    • Source: Internet
    • Author: User
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    Questions are as follows


    I would like to find pig KIT (FJ938289) on NCBI gene.the structure of the computer and the sequence of its inclusions and exons?



    % with NCBI's Spling tool, see: Illustration: How to find the sequence of the fourth exon of the HNF-4 gene on NCBI.


    % but the pig KIT (FJ938289) gene is not a Refseq sequence. This is more difficult, as can be known from the annotations of the sequence (/chromosome="8), which belongs to chromosome 8.


    % so determine whether chromosome 8 of pig (Sus scrofa) has been sequenced. First search the Tixonomy database and get the Tixonomy ID: 9823. Search the

    < database directly with the keyword >p style" "text-align: left;" >txid9823. Pigs have 19 chromosomes, resulting in 11.

    % but there is no chromosome 8. So it seems unlikely that the sequence of inclusions and exons in the pig KIT (FJ938289) gene will be determined. I can't help it anyway.


    gene structure diagram



    after the NCBI search for FJ938289, the next step is to use the NCBI provided graphics tool to view, You can know the sequence of gene inclusions and exons (and also a genetic structure diagram).

    2,GSDS Online tool: genetic structure diagram


    pig KIT (FJ938289) gene after the results of the operation of the

    < "text-align: left;" ">


    this site interface is very simple, so not much, but not smart enough.


    3, with BioPerl also drawing a genetic structure diagram


    "Read more: Bioperl: Convert the sequence in Genbank format to a genetic structure diagram

    < "text-align:left;">


    -finished. There should be other beautiful tools for drawing, but I haven't used them, so I'm not sure.


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