How to increase the amount of protease produced by strains?

: Some of my strains produce
protease
, but want specific analysis must be high purification, this is a problem? How can I increase production? Some people have asked me, since each strain will produce a series of proteases, then what can achieve commercial use of value, is any strain of bacteria as long as the separation of purified protease ok? (Compare simple's idea) Thank you all!
: There are a number of factors that affect the production of proteases in strains, including:
1, the effects of different carbon sources on enzyme production, and the effects of different nitrogen sources on enzymes
2, the carbon-to-nitrogen ratio is directly related to the growth and enzyme-producing of strains: for example, a lower carbon-to-nitrogen ratio (C) /N) is often conducive to the increase of protease yield, but under these conditions, pH of
culture
-based late fermentation tends to shift to the alkali side, which in turn inhibits the accumulation of protease, so the selection and control of the appropriate carbon-nitrogen ratio is also an important factor in increasing enzyme yield.
3, add
surfactants
Tween80 on the effect of strain-producing enzymes: low concentrations of surfactants on the growth of certain bacteria, many surfactants can also improve the production of enzymes, especially conducive to the production of extracellulase, and they do not have much specificity to bacteria and enzymes.
, surfactants commonly used to increase enzyme yield are mostly non-ionized, such as Tween80, TritonX-100, etc. They are
toxic
microorganisms, or are extremely toxic, and the enzyme-producing effect varies depending on the type of bacteria and enzymes.
4, fermentation temperature on the effect of the strain enzyme fermentation temperature can not only affect the growth of bacteria, but also affect the synthesis of enzymes. Low temperature is not conducive to the growth of strains of enzymes, with the increase in temperature, the production of enzymes of strains greatly increased, but when the temperature of more than 33 degrees C to 34 degrees C, the growth of the bacteria is the most vigorous, the enzyme vitality of strains increased significantly, to 34 degrees C to reach a peak.
from the point of view of enzyme dynamics, the temperature increases, the reaction speed increases, the growth metabolism accelerates, the production period advances. However, because the enzyme itself is very easy to lose activity due to heat, the higher the temperature, the faster the enzyme infestion, manifested in the bacteria easy to age, the shortening of the fermentation cycle, affecting the final yield of the product.
: To improve the production of protease strains, in addition to
culture base
, culture conditions to solve such problems, there is another is: you can add the appropriate amount of this protease substrate as an inducing agent to stimulate the strain to improve protease yield, culture enzyme bacteria generally use this method, so that the strain feels alive, it is willing to work hard to work hard.
the owner said the strain produces a series of proteases, the specific one or how to achieve the purpose of commercial applications, this aspect and enzyme activity related, but also with the production of this enzyme has a lot of relationship, if the yield is not high, but also very male applications in business, the current application of enzymes in business is mainly through the
gene
engineering bacteria to produce.
had previously looked up the Antarctic false silk
yeast
candida antarctica produced lipase A and lipase B two enzymes, and lipase B in the hand drug split is more widely used, so Denmark NOVOZYME will produce lipase B gene for Aspergillus. A expression, produced in the listed NOVE435 (lipase B from Candida Antarcitca), of course, also used pichia expression. That's it.
.