How to quantify multiple isomer peaks

Many pyrethroid pesticides, dimethomorphs, etc.

Cypermethrin has 4 cis-trans isomers and 8 chiral enantiomers: a pair of low-efficiency cis-forms, a pair of low-efficiency trans-forms, a pair of high-efficiency cis-forms and a pair of high-efficiency trans-forms

First, qualitatively

Second, quantitative

wi=(Ai/∑ _A )×100%×f

In the formula: w _i ——Single peak concentration of cypermethrin;

A _i ——Single peak area of ​​cypermethrin;

∑ _A ——the sum of the four peak areas of cypermethrin;

f——The actual total concentration of cypermethrin

Finally, divide the peak area of ​​the sample by the peak area of ​​the standard, and multiply it by the concentration of each peak in the standard to obtain the single peak concentration in the sample

w _i =(As/Ai)×wi

In the formula: w _s —— the single peak concentration in the sample;

A _i ——The single peak area in the standard;

A _s ——the peak area of ​​the corresponding chromatographic peak in the sample;

w _i ——The corresponding single peak concentration of cypermethrin in the standard

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