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HPLC identification of Bailing capsules
This product is a capsule made of fermented Cordyceps powder [(Cs-C-Q80) Hirsutella Sinensis Liu, Guo, Yu-et Zeng (1989) dry powder of mycelium obtained by liquid submerged fermentation]
[Identification] (1) Preparation of reference substance solution: Take an appropriate amount of adenosine reference substance, accurately weigh it, and add 0.
(2) Preparation of the test solution: take the contents of the product under the item of difference in volume, mix well, take about 0.
(3) Control medicinal material solution: Take 0.
(4) Chromatographic conditions and system suitability test: use octadecylsilane-bonded silica gel as filler; use acetonitrile as mobile phase A, use 0.
Table 19-1 HPLC conditions of Bailing capsules
Chromatographic column: Zorbax Bonus-RP, 4.
Column temperature: 25℃
Flow rate: 1.
Instrument configuration: quaternary pump with vacuum deaerator (G1354A)
Autosampler (G1367A)
Column thermostat (G1316A)
UV detector (G1314A)
Instrument control and data processing: Agilent Chemstation
Determination method: According to the four-part high-performance liquid chromatography (General Principle 0512) test of the 2015 edition of the Chinese Pharmacopoeia, draw 20ul each of the above three solutions and inject them into the liquid chromatograph
Related Links: GC Identification of Watermelon Cream Run Throat Tablets, GC Identification of Jufang Zhibao San