Human antiphospholipidase A2 antibody (Anti-PLA2R) enzyme-linked immunology analysis (ELISA)

people antiphospholipase A2antibody (Anti-PLA2R)reagents box instruction manual for research purposes only. use purpose: This kit is used to determine the content of antiphospholipase A2 antibody (Anti-PLA2R) in human serum, plasma and related liquid samples. experimental principle this kit uses indirect method to determine the level of human antiphospholipase A2 antibody (Anti-PLA2R) in the specimen. Using purified >antigen package is microplate to make solid phase antigen, to the packet of single-resistant micro-holes in turn to add a known concentration of and unknown concentration of the sample to be examined, After warmth, the biotin-labeled anti-IgG antibody is added, and then combined with streptomycin-HRP to form an immune complex, which is thoroughly washed and colored with the substrate TMB. TMB is catalyzed by HRP enzymes into blue and, under the effect of acid, into the final yellow. The depth of the color is positively related to the antiphospholipidase A2 antibody (Anti-PLA2R) in the sample. The concentration of human antiphospholipase A2 antibody (Anti-PLA2R) in the sample was calculated by using a ase marker to measure absorbance (OD value) at a wavelength of 450nm. kit composition 1. 30 times concentrated washing-up liquid 20ml × 1 bottle .."standard S1 (600ng/L) .0.5ml × 1 bottle .2 chain mycoffin -HRP .6ml × 1 bottle standard S2 (400ng/L)