Human endospinal nitric oxide synthase (eNOS) enzyme-linked immunology analysis (ELISA)

reagents for research purposes only

Purpose: This kit is used to determine humanserum, plasma and related liquid samplesendo-corted nitric oxide synthase(eNOS);

experimental principle:

< style" > kit is used in a doubleantibody clamping method to determine the specimenpeopleendoderskin nitric synthesizer(eNOS).< "color: black;" >)level. With purified human"color: black;" > endotrate-type nitric oxide synthase "> antibody package is microplate to make solid-phase antibodies, to the packet of mono-resistant micro-holes in turn added "color: " Type nitric oxide synthase (eNOS ) ", and then HRP marked "" antibody binding, forming antibody - resistance The original - ase-labeled antibody complex, after thorough washing plus substrate TMB " color. TMB is transformed into blue by the catalysis of HRP enzyme, and into the final yellow with the effect of acid. The shade of color and the < "black;" > endotred nitric oxide synthase < "span style" color: "black;" > (eNOS " " Measure the absorbance at <">450nm wavelengths" (" OD value" and calculate the "span style" in the sample by standard curve <: black;"> endo-skin nitric oxide synthase < "span style" color: "black;" > (eNOS ") concentration. < ">

" kit consists of :

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