Human endotrophic nitric oxide synthase (eNOS) enzyme-linked immunoassitric analysis (ELISA)

endoskin nitric oxide synthase (eNOS) enzyme-linked immunoanalytogenic analysis ( ELISA < "FONT-SIZE: 14.0pt;COLOR: black;">)

< "FONT-SIZE: 14.0pt;" > thebox instruction manual

reagents for research purposes only: This kit is used to determine humanserum, plasma and related liquid samplesendo-skin nitric oxide synthase (eNOS)< "FONT-SIZE: 12.0pt;COLOR: black;" >"content.

experimental principle:

< >" this kit is used in a double"> antibody sandwich method to determine the specimenpeopleendoderpine type nitric oxide synthase(eNOS ")""" With purified human"color: black;" > endotrate-type nitric oxide synthase"> antibody package ismicroplate to make solid-phase antibodies, to the packet of mono-resistant micro-holes in turn added"color: " Type nitric oxide synthase(eNOS )", and thenHRPmarked"" >eNOSantibody binds to form antibody-resistance The original- ase-labeled antibody complex, after thorough washing plus substrateTMBcolor. TMBis transformed into blue by the catalysis ofHRPenzyme, and into the final yellow with the effect of acid. The shade of color and the < "black;" > endotred nitric oxide synthase < "color: black;" > (eNOS " " Measure the absorbance at <">450nm wavelengths" (" OD value" and calculate the "span style" in the sample using the standard curve to calculate the color of endo-skin nitric oxide synthase < "span style" color: black;">"(eNOS ") concentration. < ">

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