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Human free fatty acid (FFA) enzyme-linked immunoassay

 Last Update: 2021-02-28
 Source: Internet
 Author: User

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< "FONT-WEIGHT: bold; FONT-SIZE: 14.0pt; COLOR: rgb (0,0,0); ">free fatty acids (( FFA) ase-linked immunity < "FONT-WEIGHT: bold; FONT-SIZE: 14.0pt; "> analysis

< classp" style"MARGIN-TOP: 0.0pt;MARGIN-BOTTOM: 0.0pt; TEXT-INDENT: 28.0pt; TEXT-ALIGN: center; ">reagents< "FONT-WEIGHT: bold; FONT-SIZE: 14.0pt;" > box instruction manual

reagents are for research purposes onlyPurpose: This kit is used to determine humanserum plasma and related liquid samplesfree fatty acids (FFA)content.

Experimental Principles < "FONT-WEIGHT; FONT-SIZE: FONT-SIZE: FONT-SIZE 10.5pt;">:

" This kit is< "FONT-SIZE: 10.5pt;" > dual antibodyclamping method assay< "font-SIZE: 10.5pt;" > "FONT-SIZE: 10.5pt;"in-> <"FONT-SIZE: 10.5pt;">free fatty acids (FFA)< "FONT-SIZE: 10.5pt;" > level. With purifiedfree fatty acids (FFA)antibodys pack ismicroplate to make solid phase , the package is " "Font-SIZE <: 10.5pt; COLOR: rgb (0,0,0); > free fatty acids (FFA) are added to micro-holes ," then HRP labeled free fatty acids (). FFA) antibody binds to form antibodies "Times New Roman" >- < a href" > < "Times New Roman" >- enzyme Standard antibody complex , after thorough washing < FONT-SIZE: 10.5pt; "> plus substrate <": "Times New Roman" > TMB color display. TMB at HRP enzyme catalytic conversion to blue, and under the effect of acid into the final yellow. The shade of color and the free fatty acids (FFA) is positively related. Measure the absorbent at 450 nm wavelength" ( OD value ), through the standard curve calculated sample < "FONT-SIZE: 10.5pt;"> people in the free fatty acids (FFA content.

The kit consists of :

kit consists of

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