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    Home > Biochemistry News > Biotechnology News > Human nitric oxide synthase (T-NOS) enzyme-linked immunoassay (ELISA)

    Human nitric oxide synthase (T-NOS) enzyme-linked immunoassay (ELISA)

    • Last Update: 2020-11-21
    • Source: Internet
    • Author: User
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    < "font-SIZE: 14.0pt;COLOR: black;"> nitric oxide synthase (T -NOS)immunoanalytogenic analysis ( ELISA

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    reagents for research purposes only <; "">Purpose: This kit is used to determine humanserum, plasma and related liquid samples of nitric oxide synthesis enzyme(T). -NOS)contentcontent.

    experimental principle:

    In this kit, a < a href""> antibody clamping method is used to determine the level of human nitric oxide synthase(T - <" ">NOS"level. With purified people nitric oxide synthase" (T - NOS"antibody package ismicroplate, made into solid-phase antibodies, into the packet of monoantigenic micro-holes In turn, add nitric oxide synthase" (T - < ">NOS"< """ >", and then with HRP marked nitric oxide synthase ">" (T - NOS"" antibody binding, forming antibodies - <" A href> "" - ase-labeled antibody complex, after thorough washing of the substrate TMB color. TMB is converted to blue by the catalysis of HRP and into the final yellow by the effect of acid. The shade of color and the nitric oxide synthase (T - " Measure the absorbent at <">450nm with an enzymatic labeler (" OD value"), Using the standard curve to calculate the concentration of human nitric oxide synthase " (T - " in the sample.

    " kit :

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    kit composition

    48 < ">"

    96

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