Human nitric oxide synthase (T-NOS) enzyme-linked immunoassay (ELISA)

" reagents for research purposes only < "">Purpose: This kit is used to determine humanserum, plasma and related liquid samples of nitric oxide enzymes(T -NOS)thecontent.

experimental principle:

In this kit, a < a href""> antibody clamping method is used to determine the level of human nitric oxide synthase(T - <" ">NOS"level. With purified people nitric oxide synthase" (T - NOS"antibody package ismicroplate, made into solid-phase antibodies, into the packet of monoantigenic micro-holes In turn, add nitric oxide synthase" (T - < ">NOS"< """ >", and then withHRP marked nitric oxide synthase ">" (T - NOS"" antibody binding, forming antibodies - <" A href> "" - ase-labeled antibody complex, after thorough washing of the substrate TMB color. TMB is transformed into blue by the catalysis of HRP enzyme, and into the final yellow with the effect of acid. The shade of color is positively related to the nitric oxide synthase ">" (T style"" >- Measure the absorbent at <">450nm wavelengths" ( OD values), Using the standard curve to calculate the concentration of human nitric oxide synthase " ("T - " .

" kit :

kit composition

48 < ">"

96