Human progesterone (PROG) enzyme-linked immunology analysis (ELISA)

human progesterone (PROG )enzyme-linked immuneanalysis (" ELISA ）

reagents < "FONT-WEIGHT: bold; FONT-SIZE: 14.0pt;"> box instruction manual

<:'FONT-SIZE: 12.0pt; COLOR: rgb (0,0,0); FONT-FAMILY: "Times New Roman"; '> reagents for research purposes onlyPurpose: This kit is used to determine humanserum , the cells were cleared and related liquid samplesprogesterone (PROG )content.

experimental principles :

Kits apply dual antibody clamping method assays < FONT-SIZE: 10.5pt; > specimens peopleprogesterone (). PROG )level. With purifiedpeople progesterone ( prog ) antibody pack is microplate to make solid-phase antibodies, Add progesterone ( PROG < "FONT-SIZE: 10.5pt;COLOR: rgb (0,0,0);">" ,""HRP tagged progesterone ( PROG ) ) antibody binding, forming antibody < a href"" > antigen - enzyme label antibody complex , after thorough washing plus BackgroundsTMB TMB at HRP is catalyzed into blue and converts to final yellow by acid. The shade of color and the progesterone ( >PROG ) is positively related. Font-SIZE: < 10.5pt;">450 < FONT-SIZE: 10.5pt; FONT-FAMILY: "New Times Roman"; '>nm wavelength measurement absorbent ( OD value), through the standard curve >; span style < "FONT-SIZE: 10.5pt; COLOR: rgb (0,0,0);" > progesterone ( PROG ) concentration.

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