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    Home > Biochemistry News > Biotechnology News > Human secreted phospholipase A2 (sPLA2) enzyme-linked immunoassay (ELISA)

    Human secreted phospholipase A2 (sPLA2) enzyme-linked immunoassay (ELISA)

    • Last Update: 2021-03-01
    • Source: Internet
    • Author: User
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    peopleSecretion-type phospholipase "Arial>A2( sPLA2)enzyme-linked immunityanalysis ( ELISA )

    "fontbox instruction manual

    reagents are for research purpose< > s only < "FONT-SIZE: 10.5pt;">Purpose: This kit is used to determine humanserum, plasma > and related liquid samples <secretive phospholipase A2 (sPLA2)'scontent.

    experimental principles :

    Kits apply dual"antibodyclamping method assay specimens people < "FONT-SIZE: 10.5pt;COLOR rgb (0,0,0);", "> secretion phosphatase A2 ( sPLA2) level. Using purified secretion phospholipaseAalial" >A2 ( sPLA2) antibody package is , made solid-phase antibodies, added to the single-resistant micro-holes in secretive phospholipase A2 ( sPLA2) ," then HRP "FONT-SIZE: 10.5pt;COLOR: rgb (0,0,0); ">-type secretion of phospholipidase< face"arial" > A2 ( sPLA2) antibody binds to form antibodies< "Times New Roman" >- < a href" > Antigens - enzyme-labeled antibody complex , after thorough washing plus base "Times New Roman" >TMB color. TMB at HRP is catalyzed into blue and converts to final yellow by acid. The shade of color and the people secretion type Phospholipase "Arial" >A2 ( sPLA2) is positively related. Font-SIZE: < 10.5pt;">450 < FONT-SIZE: 10.5pt; FONT-FAMILY: "New Times Roman"; '>nm wavelength measurement absorbent ( OD value), through the standard curve >; people secretion phospholipaseA2 ( sPLA2) concentration.

    " Kit composition :

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