Human thymosin α1 (Tα1) enzyme-linked immunoassay (ELISA)

).

reagents box instructions

< class Normal"style"TEXT-INDENT: 24.0pt;">reagents are for research purposes onlyPurpose: This kit is used to determine humanserum, cell serum and related liquid samples< FONT-SIZE: "FONT-SIZE" 2.0pt;">thymus peptides α1 (T α1)'s< "FONT-SIZE: 12.0pt;" > content.

experimental principle:

<" > antibodyclamping method to determine the human in the specimenthymus peptides α 1 ( T α 1 )"level. With purified people thymus peptides α1( T α 1 )" < > antibody package ismicroplate", made into solid antibodies, added to the packet of mono-resistant micro-holes in turn thymus peptides α 1 ( T α 1 < Span style: "color: black;" >) ", and then with HRP α 1 ( T α 1 ) antibody binding, forming antibody - antigen - "">ase-labeled antibody complex, after thorough washing of the substrate TMB color. TMB is transformed into blue by the catalysis of HRP enzyme, and into the final yellow with the effect of acid. The color of the depth and sample of thymus peptide α 1 ( α 1 """"""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""" Measure the absorbance at "" <450nm

wavelengths" ( OD "" and calculate the human > thymus peptides α < < >αspan style="color;">1 ":"black;">" <"""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""" 1 ) concentration."

< "> kit consists of :

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kits make up

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