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Identification of HR-Inducing cDNAs from Plant Pathogens via a Gateway-Compatible Binary Potato Virus X-Expression Vector

 Last Update: 2021-03-13
 Source: Internet
 Author: User

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dna

dna expression

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Identification of pathogen effectors that elicit a hypersensitive response (HR) in resistant plant hosts is essential to study disease resistance. In this method, it is described how to generate a c
DNA
library, how to transfer the library into a binary PVX-expression vector, and finally how to set up a high-throughput screen for HR-inducing
cDNA
s from plant pathogens.

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