Identification of nucleotides.

general identification reaction of glycosides
1. Preparation of the inspection solution:
Herbal water immersion: take Chinese herbal fragments or powder 2g, plus distilled water about 20ml 70C± water bath, impregnated for 10 minutes,
filtration
, filter for identification.
Chinese herbal alcohol leaching solution: take Chinese herbal fragments or powder a little less in the
tub tube
, add 10 ml of ethanol, soak in a warm bath for 10 minutes, filtration, filter for identification.
2. Identification test:
(1) - Molish reaction take alcohol immersion 1 ml, plus 10% - phenol liquid 1 drop, shake well, slowly add thick H2SO4 10 drops along the pipe wall, shake, observe whether the two liquid interface between the purple cup (this reaction detects sugar, glycosides
compounds
, the reaction is more sensitive. If a trace amount of
filtration paper
fiber or herbal powder is present in the solution, can produce the above reaction, so should be noted when filtration).
(2) hydrolysing reaction:
water immersion 3 ml in the test tube, plus 10% HCl 1 ml on the boiling water bath
heat
20 minutes, to see if there is floccular precipitation produced?
(3) alkaline brass acid (Filin
reagent
Fehling's test solution) test
take 2 ml of leachate and add the newly-provisioned filin reagent (A) 1 ml, heated on the boiling water bath for a few minutes, such as the production of red copper oxide precipitation, then line filtration, filter liquid plus 10% HCl adjusted to acidity, heated on the water bath for 10 minutes. Hydrolysing and filtering out if there is floc-like precipitation.
then add 10% NaOH to the medium and add the Filin reagent 1 ml, still heating in a boiling water bath for 5 minutes to see if yellow, brick red or brown precipitation is produced? (This reaction test polysaccharides, nucleotides) from the reaction results to show whether the herbal medicine in the test contains glycosides?
(This test method can also use the same volume of the same concentration of Chinese herbal leaching two parts, one first by acid hydrolytic filtration alkalinization, the other at the same time to carry out the reduction reaction, compared with the amount of copper oxide generated, whether there is a difference between the two parts to judge, the specific method can be seen in the system pre-test experiment).)
: The general identification of chinese herbal medicine to glycosides is positive, and further identification of individual gsides can be made. The specific method is as follows.
.