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Methods for the identification of proteins have advanced dramatically this decade through the introduction of mass spectrometric techniques and instrumentation sensitive enough to be applicable to biological systems (
1
). The two mass spectrometric techniques that have provided these advantages are electrospray-ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) (
see
Chapters 52 , 54 , and 55 ).