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    Home > Biochemistry News > Biotechnology News > Identification of Regulatory Protein- Binding Sites in Cytochrome P450 Genes by Means of the Gel- Retardation Assay

    Identification of Regulatory Protein- Binding Sites in Cytochrome P450 Genes by Means of the Gel- Retardation Assay

    • Last Update: 2020-12-23
    • Source: Internet
    • Author: User
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    The gel-retardation, band-shift, or electromobility shift assay is used to investigate protein—
    DNA
    interactions. Nuclear protein from the desired source is incubated with a radiolabeled DNA fragment. The products of the reaction are then analyzed by electrophoresls through a nondenaturing polyacrylamide gel. Free DNA will migrate rapidly through the gel, but DNA bound to protein will move more slowly, i.e., the migration of bound DNA ts retarded. The protein-DNA complexes formed can be detected by autoradiography.
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