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Identification of Regulatory Protein Genes Involved in Alkaloid Biosynthesis Using a Transient RNAi System

 Last Update: 2021-01-18
 Source: Internet
 Author: User

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RNAi (RNA interference, RNA silencing) is a powerful tool in functional genomics. We report here the use of transient RNAi to isolate regulatory factor genes involved in isoquinoline alkaloid biosynthesis in
Coptis japonica
protoplasts. Double-stranded (ds) RNAs prepared against candidate regulatory factors, which were predicted from an EST library, were introduced into
C. japonica
protoplasts by polyethylene glycol (PEG)-mediated transformation, and their effects were monitored by real-time reverse transcription (RT)-polymerase chain reaction (
PCR
). The potential of this transient RNAi system to characterize the functions of regulatory factor genes in alkaloid research is discussed.

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