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    Home > Biochemistry News > Plant Extracts News > Identification of Regulatory Protein Genes Involved in Alkaloid Biosynthesis Using a Transient RNAi System

    Identification of Regulatory Protein Genes Involved in Alkaloid Biosynthesis Using a Transient RNAi System

    • Last Update: 2021-01-18
    • Source: Internet
    • Author: User
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    RNAi (RNA interference, RNA silencing) is a powerful tool in functional genomics. We report here the use of transient RNAi to isolate regulatory factor genes involved in isoquinoline alkaloid biosynthesis in
    Coptis japonica
    protoplasts. Double-stranded (ds) RNAs prepared against candidate regulatory factors, which were predicted from an EST library, were introduced into
    C. japonica
    protoplasts by polyethylene glycol (PEG)-mediated transformation, and their effects were monitored by real-time reverse transcription (RT)-polymerase chain reaction (
    PCR
    ). The potential of this transient RNAi system to characterize the functions of regulatory factor genes in alkaloid research is discussed.
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