Identification of steroids.

1. Preparation of the inspection solution
take five times (including hydrolytic terrification), children's tea (containing lysate), no food acid (hydration hydrolyzed pseudo-ternification) a small amount (about 0.1 g) are placed in large
test tubes
respectively, with distilled water about 10 ml,
heating
boiling,
filtration
, filter for the following test:2. The general reaction of tanning (difference between tannin and pseudo-ion technique identification)
(1) sensory test: take prepared tannin and pseudo-icing solution, taste its taste, and check whether the solution is acidic with stone
test paper
(2) Iron triclosan reaction: the preparation of tantaclic solution (five times sub-solution or tea solution) and pseudo-tantapic solution (food acid solution) each 1 to 2 ml, respectively, added iron triclosan test solution, tantacerine produces green or blue-black reaction or precipitation;(3) precipitation protein reaction: take the technology solution and pseudo-tin solution each 1 to 2 ml, respectively, into the gelatin solution drops, the technology immediately produces precipitation reaction.(4) Alkaloid reaction: take 1 to 2 ml of tanning solution and pseudo-ion solution, respectively, drop plus 0.1% coffee alkaline water solution, espresso liquid produces precipitation reaction, pseudo-amine does not produce precipitation reaction.3. The difference between hydrolytic tanning and condensed tanning is identified
(1) redness reaction: take five times the sub-immersion (containing hydrolyzed tantasium), the tea leaching solution (containing the condensed tantaclic) each 2 ml, respectively, plus hydrochloric acid 0.5 ml, heating and boiling for about 30 minutes to cool. Hydrolytic tanning does not occur precipitation, and the shrinking tanning has red precipitation.(2) Iron triclosan reaction: take five times the sub-immersion and tea immersion flow of 1 to 2 ml each, respectively, add the number of iron triclosan test drops, hydrolyzed tannins blue or black or blue reactions, shrinking tannins appear black-green reactions.(3) Bromine water reaction: take five times the sub-immersion with children's leaching solution each 1 to 2 ml, respectively, added several drops of bromine water, hydrolytic
shoe hat
quality does not produce precipitation reaction, shrinking tantacopic precipitation.(4) lime water reaction: take five times the sub-immersion and tea leaching liquid each 1 to 2 ml, respectively, add a number of drops of newly made lime water, can hydrolyzed tantails appear green gray precipitation, shrink tanning brown precipitation.(5) Acetic acid solution and lead acetate reaction: take five times the sub-immersion and tea leaching liquid each 1 to 2 ml, respectively, add acetic acid drops, shake well and then drop the lead acetate solution drop a few drops, hydrolytic tantail produces floc-like precipitation, shrinking phosphonate without precipitation generation..