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Immunization Protocol for Monoclonal Antibody Production

 Last Update: 2020-10-26
 Source: Internet
 Author: User

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Immunization Protocol for Monoclonal Antibody Production

Antigens:

Proteins: 10-100 µg

Adjuvant:

Complete Freunds Adjuvant

Schedule:

Day	Manipulation </ P>	Adjuvant	Site
0	Primary Immunization	CFA	s.c.
14	Boost #1	IFA	s.c.
24	Boost #2	IFA	s.c.
35	Boost #3	IFA	i.v.
42	Bleed and test for antibody
45	Boost #4	IFA	i.v.
66+	3-4 week rest followed by i.v. or i.p. boost	Saline/PBS	i.v.
69	fusion 3d after i.v. boost or 4d after i.p. boost	�	i.v.

：Polyclonal Antibody Production：The production of monoclonal antibody by hybridoma

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