Immuno-affinity and layering techniques.

affinity and layering technology is an effective method for purifying various biomodynists.
Antibody
was originally used as a binding agent for immunopromising layering, collecting antigens based on the principle of
antigen
antibody binding to form a large number of polymers and insoluble substates. Subsequently, the use of antibodies and inert microbeads co-priced cross-linking, although this reaction is a simple binding, but the activity of antibodies due to cross-link lack of positioning action or excessive cross-linking of antibodies and lost.found that antibodies are more likely to bind to antigens after co-priced co-linking with protein A or protein G microbeads. It has become a practical and effective purification method to make various monoclonal antibodies with good antigen binding characteristics and a wide range of sources into immuno-affinity column.immuno-affinity layering has the following characteristics:
(1) antibody binding with its corresponding antigen has a high affinity and specificity, can be a large number of separation of natural state or similar natural state of antigen.
(2) not all antibodies are suitable for immuno-affinity, but once a well-performing antibody is obtained, the purification process is simple, fast and reliable.
(3) can be carried out on different scales, can be completed in half a day, and can obtain other layering methods incomparable purification effect.
(4) has been simplely improved, immuno-affinity can also be used to purify specific antibodies against antigens.
immune affinity is a simple and very effective technique for separating antigens. The antibody co-price is combined to an inert microbead, which is then mixed with a solution containing antigens to be purified. When the antigen is cross-linked to the antibody on the microbeads, the unrelated antigen is removed by washing, and then the microbeads are treated with a wash-out buffer, and the combined antigen is purified.purified antigens can still maintain their natural state if the conditions for purification are better and milder. Although all of the following examples are based on
protein
antigens, any molecule that binds effectively to antibodies can be purified in this way.simply change the operating procedure, immuno-affinity and layering can also be used to isolate initially purified antibodies. At this time antigen and antibody play the opposite role, antigen co-price crosslink on the microbeads, and then bind to the antibody, and then by purification, to obtain purified antibodies.conditions, the application of immuno-affinity and layering method usually only need one time to achieve 1000 to 10000 times the purification effect. The use of particularly good performance of antibodies, and master the conditions for purification, can also reach more than 10000 times the purification effect..