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Immunological Detection of Gene Expression in Recombinant Clones

 Last Update: 2021-01-06
 Source: Internet
 Author: User

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rna situ hybridization

gene

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After ligation and transformation, a number of clones will (it is hoped) be obtained that can be identified as recombinants by the occurrence of insertional inactivation, by analysis of small-scale plasmid preparations, or more specifically by
in situ
hybridization. An additional strategy that is superficially attractive, but not without considerable problems, is to look directly for expression of the cloned gene(s). This may be applicable in primary cloning if no specific
DNA
probe is available, but is more generally used in subsequent studies of expression of specific foreign genes.

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