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    Home > Biochemistry News > Biotechnology News > Improve the efficiency of pig cloning: pig cloning degraded embryos and XIST gene knockout pig.

    Improve the efficiency of pig cloning: pig cloning degraded embryos and XIST gene knockout pig.

    • Last Update: 2020-08-13
    • Source: Internet
    • Author: User
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    Recently, the latest research results of the Lai Liang's Research Group of the Guangzhou Institute of Biomedicine and Health of the Chinese Academy of Sciences, based on XIST Derepression in Active X Hinders Pig Somatic Cell Nuclear Transfer, were published online in STEM CELL REPORTS."
    the study for the first time, the study explores the effects of long-chain non-coding RNA XIST and histone H3K9me on X chromosomes on the development of pig cell cloning embryos, and intervenes in the expression level of cloned embryos to greatly improve the efficiency of pig cloning.
    self-cloning sheep "Dolly" produced, cloning technology after decades of development, but including pigs, the efficiency of cloning large animals is still very low.
    Lai Liang's research team found in years of cloned pig research, pig cloned embryos transplanted to a surrogate mother, most of the fetus will be degraded, the mechanism is not clear.
    the team, Dr. Zhai Degong, and other experts from the analysis of normal-developed fetal and degenerative absorption of the fetal expression spectrum, found that the gene expression of cloned embryos in pigs and mice significantly different, mouse cloned embryo gene reduction mainly occurred in the X chromosome, and pig cloned embryo gene reduction occurred on all chromosomes.
    researchers used gene editing technology to target the inactivated XIST gene, which can save the downgraded gene, thereby improving the in vitro development quality and potential of the pig cloned embryo, and increasing the efficiency of cloning of pigs by about seven times.
    further found that the developmental ability of XIST knockout cloned embryos was closely related to the level of histone H3K9me3 in the embryo.
    researchers actively reduce the level of H3K9me3 in cloned embryos, can also significantly improve the development efficiency of early cloned embryos in pigs, but actively lowering the level of H3K9me3 will relieve its inhibition effect on XIST, induce the abnormally high expression of XIST gene in cloned embryos, therefore, by injecting H3K9me3 demethylase into pig cloned embryos to reduce H3K9me3 levels, and not improve the efficiency of cloning in pigs.
    the research results to some extent, the special mechanism of pig cell cloning process, in order to improve the efficiency of pig cloning provides a new way of thinking.
    research work has been supported by the National Natural Science Foundation of China, the Chinese Academy of Sciences international cooperation projects, the Guangdong Science and Technology Program project and the Guangzhou Science (Technology) Research Project.
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