In-body production of red blood cells helps solve the world's problems of blood shortage

Science and Technology Daily, Hefei, February 3 (Reporter Wu Changfeng) reporter from the University of Science and Technology of China was informed that the university's Department of Life Sciences and Medicine Professor Cheng Linyi led the task force and cooperation team, research and establishment of a large number of extraterrocity of individual nuclear cells from human blood red system The experimental system of the ancestral cells and the efficient induction of differentiation into mature red blood cells has been found for the first time that the exogenetic expression of the BMI1 gene can promote the insular amplification of the red line ancestral cells by up to one trillion times, which provides a new way of thinking to solve the worldwide problem of red blood cell shortage. The findings were published in molecular therapy, the journal of the American Society for Gene and Cell Therapy. Internationally renowned blood experts commented that this work "is a breakthrough in the field of blood transfusion, to solve the bottleneck of blood shortage."
present, red blood cells and other blood products mainly come from volunteer exo-weekly blood donation, insufficient donors, infection risk, rare blood type deficiency, etc. are still the world's blood transfusion problems. In-body induced erythrogenic stem cell targeted differentiation into red blood cells is another important way to solve the above problems, but the erythylocyte dekernel efficiency of the source of erythmatic stem cell differentiation is very low, it is difficult to produce enough mature red blood cells. How to obtain a large number of functional red blood cells through in-body culture is an important challenge in this field.
through a detailed analysis of a single nuclear cell-rich CD235a plus red line ancestral cells of the healthy provider's outer blood, the researchers found that the red line ancestral cells could not maintain the state of self-renewal for a long time, and rapidly differentiated and then apoptosis. High-volume sequencing results show that, with the differentiation of in-body culture of red-line progenitor cells mature, the expression of the BMI1 gene is rapidly reduced, and the exogenetic over-expression of BMI1 can enable the red-line progenitor cells to maintain their ability to self-renewal, expanding continuously in the culture system, and can be expanded up to 1 trillion times in 2 months. In addition, the study found that BMI1 had the same effect on red line ancestral cells from patients with sickle anemia. Mature red blood cells induced to deuterucleosis were injected into the blood transfusion model mice through tail intravenous injections, and it was found that human red blood cells from cultured sources had the same in vivo circulation function as normal exodus red blood sources. Finally, the team also confirmed that in-body amplification of red line ancestral cells can be further genetically engineered to meet a wider range of precision medicine.