In D2-Chip, the experimental study of bias-random-walk motion and the effects of deified memory was completed at the same time.

Recently, Liu Yong, a researcher at the Institute of Applied Technology at the Hefei Institute of Material Sciences of the Chinese Academy of Sciences, worked with Francis Lin, a professor at the University of Manitoba in Canada, to make new progress in cell-based cell chemistry and memory effects based on microflow-controlled chip technology, and the results were published in the journal Integorative Biology (2017, DOI:10.1039/C7IB00037E).
cell chemization refers to the directional migration movement of cells towards a specific chemical concentration gradient.
Previous studies have detailed how neutral granulocytes respond to chemical concentration gradients and simulated neutral granulocyte chemical motion using bias-random-walk theory, but the experimental validation has not yet been completed due to the highly persistent migration properties of cells.
another issue that has been of concern in the field of cell migration is the cell-to-cell-called memory effect and its potential mechanisms.
Relying on a microflow control chip with cell pre-calibration and multi-region gradient configuration, the team developed a double-thin-walled barrier microflow control chip (Double docking chip, D2-Chip) for this research.
D2-Chip consists of an S-type microchannel structure, a detection area, and a downstream channel structure (Figure 1A).
gradient distribution in D2-Chip (Figure 1A) was simulated using multiphysics simulation software, and the modeling results show that there are two linear concentration gradients with different slopes in the intermediate gradient channel and thin-walled barrier structure (Figure 1B). After
is injected into the cell, the pressure difference between the intermediate gradient channel and the Source/Sink Channel pushes the cells to both sides of the intermediate gradient channel, and the cells are arranged on both sides of the intermediate gradient channel to give them the same initial position, a unique design that allows you to observe the cell's movement over time.
results show that cell dependence on chemical attraction gradients decreases over time, while statistical changes over time lead to reduced cell synchrotronity (Figure 1C), which is consistent with computer simulation results.
In the same group of experiments, the researchers also completed a study of the memory effects of neutral granulocytes, which showed that cells continued to move a distance in a gradient-free environment after passing through a thin-walled barrier structure (Figure 1C).
In recent years, the biomedical optical research team of photoelectronics center has carried out research on nucleic acids, proteins and cell detection based on microflow control chips for the needs of the health industry, developed a series of microflow control chips and detection devices for gene testing and cell migration research, and is working with several research teams at home and abroad for POCT applications, and is conducting new research on biomarker detection technology based on microflow control chips and smartphones.
.