Regenerative medicine therapy based on stem cells is the most promising method for treating major difficult diseases caused by human tissue, organ defects and lesions, and has been successful in clinical treatment research on bone, heart, liver, eye and other tissue repair.
the success of stem cell regenerative medicine requires us to clarify the distribution, survival and differentiation behavior of transplanted stem cells in the body, as well as the corresponding side secretion function.
to understand the behavior of transplanted stem cells in living bodies in order to improve the efficacy and safety of stem cell therapy, is currently a key problem to be solved in the clinical transformation of stem cell therapy.
therefore, it is of great significance to develop the live imaging technology that can realize the in situ tracing of the distribution, survival and differentiation of transplanted stem cells in the living body, which will greatly promote the development of efficient and safe stem cell therapy and its clinical transformation.
optical imaging technology has the unique advantages of high imaging speed, high sensitivity and multi-channel imaging, and is one of the most important imaging methods in biomedical research.
, near-infrared optics (700-1700 nm) imaging technology has been widely used in stem cell tracers because of its effective ability to reduce the absorption and scattering of light in tissues to improve the depth of tissue penetration and spatial resolution of fluorescence imaging.
especially with the development of nanotechnology, the development of a series of near-infrared fluorescent nanoprobes with different compositions, different optical characteristics and different functions has greatly improved the tissue penetration depth, sensitivity, time resolution, spatial resolution and signal-to-noise ratio of fluorescence imaging.
, the application of near-infrared two-zone (NIR-II, 1000-1700 nm) fluorescence imaging technology with very low tissue absorption, scattering, and spontaneous fluorescence properties of tissue is opening a more accurate live "visual" optical window for people to understand the behavior of stem cells.
recently, Wang Qiangbin, researcher of the Suzhou Institute of Nanotechnology and NanoBionics of the Chinese Academy of Sciences, in its invitation review of "Advanced Advances in The Track The Track The Stem Cells Fluorescent Fluorescent Nanoprobes: Turning From The First to The Second Near Near-Near-infrared Fluorescent Nanoprobe" on stem cell tracing in recent years The research progress is summarized, and the development, characteristics and stem cell tracing applications of different types of near-infrared fluorescent nanoprobes, such as fluorescent quantum dots, rare earth luminescence probes, organic fluorescent nanoprobes and multimode fluorescent nanoprobes, focusing on the development and application of near-infrared two-zone optical imaging nanoprobes represented by Ag2S quantum dot probes are analyzed.
finally, the development of ultra-high-sensitive probes to monitor the behavior of individual stem cells, monitoring the specific functions and behavior of stem cells functional probes, while analyzing the multiple behavior of stem cells multi-modal probes and simultaneous monitoring and regulation of stem cell behavior fluorescent nanoprobes.
review article spublished online in Advanced Healthcare Materials (DOI: 10.1002/adhm.201800497).