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In reducing blood lipids and improving fatty liver, which one of these two local black teas is better?

 Last Update: 2022-04-24
 Source: Internet
 Author: User

Effects of dietary black tea on visceral fat and serum lipids

UPLC-Q-TOF/MS analysis showed differences in the content of phenolic acids, catechins and flavonoids between KBT and DBT .
To elucidate the differences in the anti-obesity effects of KBT and DBT in HFD-fed mice, body weight gain and visceral fat mass in experimental animals were measured .
As shown in Figure 2A, there was no significant difference in initial body weight among the four groups .
After the third week, the HFD-treated mice had significantly higher body weights than the LFD-treated mice, and they ended up gaining 81.
4% more body weight than the LFD-treated mice .

Dietary DBT significantly reduced body weight by 30.
7% .
Although body weights of HFKB-treated mice showed a downward trend at week 15 compared with HFD mice, no significant differences were observed (food consumption of the four groups was comparable throughout the experimental period) .
HFD significantly increased visceral fat mass, and HFKB and HFDB treatment significantly reduced perirenal fat mass deposition by 22.
8% and 32.
8% , respectively (Table 2) .
There were no significant differences in mesenteric and epididymal fat mass between the HFD , HFKB, and HFDB groups .
Likewise, HFD significantly increased levels of lipid parameters (LDL-C, HDL-C, and TC), whereas black tea treatment failed to prevent the elevation of these parameters .
In addition, the serum TG levels of the four groups of mice were comparable .

UPLC-Q-TOF/MS analysis showed differences in the content of phenolic acids, catechins and flavonoids between KBT and DBT .

Effects of dietary black tea on the development of fatty liver

The effect of dietary black tea on the development of fatty liver The effect of dietary black tea on the development of fatty liver

As can be seen from Table 2, HFD significantly increased liver weight, and black tea treatment slightly decreased but not significantly decreased the total liver weight/body weight ratio

As can be seen from Table 2, HFD significantly increased liver weight, and black tea treatment slightly decreased but not significantly decreased the total liver weight/body weight ratio
.

Liver histopathological analysis was performed using H&E stained sections
.

Effects of dietary supplementation of black tea on total bile acids and lipids in feces

To investigate the effect of dietary black tea on intestinal fat absorption, total bile acid and lipid levels were measured in mouse feces

To investigate the effect of dietary black tea on intestinal fat absorption, total bile acid and lipid levels were measured in mouse feces
.

Effects of dietary black tea on gene expression of lipid metabolism in liver

To further study the preventive effect of dietary black tea on excessive hepatic fat deposition, the expression of lipid metabolism genes in the liver was determined
.
DBT significantly reduced hepatic HMG-CoA reductase (HMGR) mRNA levels, neutralizing the effects of HFD treatment (Fig.
4A)
.
However, both black tea treatments did not alter stearoyl-CoA desaturase 1 (SCD1), fatty acid synthase (FAS), sterol regulatory element binding protein 1c (SREBP1c), acetyl-CoA carboxylase A (ACACA) and mRNA expression of acetyl-CoA carboxylase B (ACACB; Figure 4)
.
The expressions of SCD1, FAS, ACACA and ACACB were comparable among the four groups
.
Notably, HFD significantly increased hepatic lipoprotein lipase (LPL) mRNA levels and suppressed the expression of adipose triglyceride lipase (ATGL); black tea treatment completely reversed HFD -induced changes in these two genes
.
Compared with the HFD group, the expression levels of peroxisome proliferator-activated receptor alpha (PPARα), carnitine palmitoyl transesterase 1 (Cpt1α) and acyl-CoA oxidase (ACOX) genes in the black tea group were significantly enhanced (Figure 4B)
.
Unlike the effect of alleviating fatty liver, KBT or DBT treatment had no significant difference in the changes of mRNA expression of hepatic lipid metabolism genes
.

Effects of dietary black tea on the expression of genes related to lipid transport and metabolism in the small intestine

Given the significant effect of dietary black tea in promoting fecal lipid excretion, the mRNA expression levels of genes involved in small intestinal lipid transport and metabolism were quantified
.
HFD significantly increased ACAT2, Cpt1α and ACOX mRNA levels and decreased gene expression of atp-binding cassette subfamily g5 (ABCG5), ATGL and Npc1l1 ( Fig.
4c)
.
In addition, DBT treatment significantly decreased the expression of ACAT2 gene and increased the mRNA levels of ABCG5 and ATGL, indicating that DBT treatment has a neutralizing effect on the changes caused by high-fat feeding
.
Changes in these genes showed similar trends by KBT treatment; however, no significant differences were observed
.
None of the black tea treatments changed the expression of Npc1l1 gene
.
Furthermore, mitochondrial functional protein (MTP) and atp-binding cassette transporter A1 mRNA levels were comparable among the four groups (Fig.
4C)
.

Effects of dietary black tea on gut microbiota

The gut microbiota is closely associated with HFD-induced obesity and nonalcoholic fatty liver disease (NAFLD)
.
Fecal SCFA concentrations were analyzed using gas chromatography, and colonic microbiota was analyzed using 16S rDNA gene sequencing
.
The data showed that high-fat feeding significantly reduced total SCFA content, acetate, propionate, and butyrate; however, neither black tea treatment prevented the feeding-induced changes
.
Chao 1 and ACE estimators and Shannon and Simpson indices were used to assess community richness and diversity
.
Chao 1 and ACE estimates or Shannon and Simpson indices were not significantly different between the four groups
.
However, HFD caused dramatic changes in the gut microbiota
.
Compared with LFD animals, HFD mice had an increased proportion of Firmicutes and a decreased proportion of Bacteroidetes, and the ratio of Firmicutes to Bacteroidetes was significantly increased in HFD mice
.
However, black tea treatment did not significantly affect this ratio
.
Likewise, in the results of principal coordinates analysis and linear discriminant analysis effect size, black tea supplementation had little effect on the modulation of gut microbiota (Fig.
5)
.

The gut microbiota is closely associated with HFD-induced obesity and nonalcoholic fatty liver disease (NAFLD)
.
Fecal SCFA concentrations were analyzed using gas chromatography, and colonic microbiota was analyzed using 16S rDNA gene sequencing
.
The data showed that high-fat feeding significantly reduced total SCFA content, acetate, propionate, and butyrate; however, neither black tea treatment prevented the feeding-induced changes
.
Chao 1 and ACE estimators for nonalcoholic fatty liver disease and Shannon and Simpson indices to assess community richness and diversity
.
Chao 1 and ACE estimates or Shannon and Simpson indices were not significantly different between the four groups
.
However, HFD caused dramatic changes in the gut microbiota
.
Compared with LFD animals, HFD mice had an increased proportion of Firmicutes and a decreased proportion of Bacteroidetes, and the ratio of Firmicutes to Bacteroidetes was significantly increased in HFD mice
.
However, black tea treatment did not significantly affect this ratio
.
Likewise, in the results of principal coordinates analysis and linear discriminant analysis effect size, black tea supplementation had little effect on the modulation of gut microbiota (Fig.
5)
.

In conclusion, most of the compounds that distinguish KBT from DBT are phenolic compounds, theanine and D-psicose
.
DBT was more effective than KBT in preventing excess fat accumulation in the liver of mice on a high-fat diet .
Both black tea treatments were effective in altering liver mRNA levels of genes involved in cholesterol synthesis, fat lipolysis, fatty acid beta oxidation, and absorption of free fatty acids and cholesterol from the circulation .
DBT treatment exhibited a more favorable effect than KBT treatment in stimulating fecal fat excretion, which may be a major factor in the different health-promoting effects of the two tea treatments in this study .

In conclusion, most of the compounds that distinguish KBT from DBT are phenolic compounds, theanine and D-psicose
.
DBT was more effective than KBT in preventing excess fat accumulation in the liver of mice on a high-fat diet .
Both black tea treatments were effective in altering liver mRNA levels of genes involved in cholesterol synthesis, fat lipolysis, fatty acid beta oxidation, and absorption of free fatty acids and cholesterol from the circulation .
DBT treatment exhibited a more favorable effect than KBT treatment in stimulating fecal fat excretion, which may be a major factor in the different health-promoting effects of the two tea treatments in this study .
In conclusion, most of the compounds that distinguish KBT from DBT are phenolic compounds, theanine and D-psicose
.
DBT was more effective than KBT in preventing excess fat accumulation in the liver of mice on a high-fat diet .
Both black tea treatments were effective in altering liver mRNA levels of genes involved in cholesterol synthesis, fat lipolysis, fatty acid beta oxidation, and absorption of free fatty acids and cholesterol from the circulation .
DBT treatment exhibited a more favorable effect than KBT treatment in stimulating fecal fat excretion, which may be a major factor in the different health-promoting effects of the two tea treatments in this study .

Original source:

Liao W, Liu S, Chen Y, et al.
Effects of Keemun and Dianhong Black Tea in Alleviating Excess Lipid Accumulation in the Liver of Obese Mice: A Comparative Study.
Front Nutr .
2022;9:849582.
Published 2022 Mar 15.
doi :10.
3389/fnut.
2022.
849582

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