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In situ
hybridization is a method used to localize nucleic acid sequences in cells or tissue sections. The first
in situ
hybridization experiments were described by two groups of researchers in 1969, John et al. and Gall and Pardue (reviewed in ref.
1
). Radioactively labeled RNA probes were used to localize specific
DNA
sequences within the nuclei of
Xenopus laevis
(
2
).