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In Vitro Continuation of RNA Synthesis Initiated in Vivo

 Last Update: 2021-03-04
 Source: Internet
 Author: User

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Isolated nuclei will continue synthesis of RNA initiated in vivo, but reinitiation of synthesis is rare in washed nuclei (
1
). This situation can be exploited to measure instantaneous rates of in vivo transcription because the cell-free conditions are well-defined and nascent transcripts are generally not subject to the rapid cleavage often found in living cells (
2
–
5
). Isolated nuclei can also be used to map a primary transcript on genomic
DNA
. The method has been used to show that transcription terminates more than 1000 nucleotides downstream from the poly A site in (β-major globin mRNA (
6
).

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