echemi logo
Product
  • Product
  • Supplier
  • Inquiry
    Home > Biochemistry News > Biotechnology News > In Vivo Labeling and Analysis of Mitochondrial Translation Products in Budding and in Fission Yeasts

    In Vivo Labeling and Analysis of Mitochondrial Translation Products in Budding and in Fission Yeasts

    • Last Update: 2020-12-12
    • Source: Internet
    • Author: User
    Search more information of high quality chemicals, good prices and reliable suppliers, visit www.echemi.com

    Mitochondrial biogenesis requires the contribution of two genomes and of two compartmentalized protein synthesis systems (nuclear and mitochondrial). Mitochondrial protein synthesis is unique on many respects, including the use of a genetic code with deviations from the universal code, the use of a restricted number of transfer RNAs, and because of the large number of nuclear encoded factors involved in assembly of the mitochondrial biosynthetic apparatus. The mitochondrial biosynthetic apparatus is involved in the actual synthesis of a handful of proteins encoded in the mitochondrial
    DNA
    . The budding yeast
    Saccharomyces cerevisiae
    and the fission yeast
    Schizosaccharomyces pombe
    are excellent models to identify and study factors required for mitochondrial translation. For that purpose,
    in vivo
    mitochondrial protein synthesis, following the incorporation of a radiolabeled precursor into the newly synthesized mitochondrial encoded products, is a relatively simple technique that has been extensively used. Although variations of this technique are well established for studies in
    S. cerevisiae
    , they have not been optimized yet for studies in
    S. pombe
    . In this chapter, we present an easy, fast and reliable method to
    in vivo
    radiolabel mitochondrial translation products from this fission yeast.
    This article is an English version of an article which is originally in the Chinese language on echemi.com and is provided for information purposes only. This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed description of the concern or complaint, to service@echemi.com. A staff member will contact you within 5 working days. Once verified, infringing content will be removed immediately.

    Contact Us

    The source of this page with content of products and services is from Internet, which doesn't represent ECHEMI's opinion. If you have any queries, please write to service@echemi.com. It will be replied within 5 days.

    Moreover, if you find any instances of plagiarism from the page, please send email to service@echemi.com with relevant evidence.