Institute of Aquatic Studies finds that decabromodiphenyl ethane affects muscle contraction and reproductive endocrine system in female zebrafish

Recently, Zhou Bingsheng's team from the Institute of Hydrobiology, Chinese Academy of Sciences published a research paper online entitled " Decabromodiphenyl ethane (DBDPE) mainly affected the muscle contraction and reproductive endocrine system in female adult zebrafish " in Environmental Science & Technology , an authoritative journal in the field of environmental science .

In this study, researchers exposed zebrafish females to DBDPE (0, 1, 100 nM)

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The researchers also performed TMT quantitative proteomic analysis of brain tissue and validated differential proteins combined with parallel reaction monitoring (PRM) technology

Figure 2 Quantitative proteomic analysis shows that DBDPE mainly affects muscle contraction and egg fertilization and other processes 

The above toxic effects of DBDPE on zebrafish were further verified: (1) The intracellular calcium homeostasis of zebrafish brain was disrupted, and the movement distance and speed of zebrafish were significantly decreased, indicating that DBDPE may affect calcium ions by affecting Changes in signaling pathways and muscle-related proteins affect muscle contraction function, thereby causing changes in zebrafish motor behavior; (2) The transcription levels of genes related to the hypothalamic-pituitary-gonadal-liver (HPGL) axis change, estrogen and testosterone The level of DBDPE decreased significantly, indicating that DBDPE may inhibit the synthesis of steroid hormones by inhibiting the expression of gnrh3 , fshb , star

Figure 3 Effects ofDBDPE on muscle contraction and reproductive endocrine-related indicators

Using zebrafish as a model organism, this study used TMT proteomics and PRM technology, combined with multiple levels of toxicity endpoint validation, and found and confirmed for the first time that DBDPE exposure affects the muscle contraction and reproductive endocrine system of female zebrafish.

Researcher Zhou Bingsheng and associate researcher Yang Lihua of the Institute of Hydrobiology are the corresponding authors of the paper, and Sun Yumiao, a doctoral student of the Institute of Hydrobiology, is the first author of the paper