Introduction: Cell-Based Assays for High-Throughput Screening
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Last Update: 2021-02-28
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Source: Internet
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Author: User
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Cell-based assays represent approximately half of all high-throughput screens (HTS) currently performed. Here we review thehistory and status of HTS, and summarize some of the challenges and benefits associated with the use of cell-based assaysin HTS, drawing upon themes that will reemerge in subsequent chapters in this book. Approaches for successful experimentaldesign and execution of cell-based HTS are introduced, including strategies for assay development, implementation of primaryand secondary screens, and target identification. In doing so, we hope to provide a comprehensive review of the cell-basedHTS process and an introduction to the methodologies and techniques described in this book.
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