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Invader Assay for Single-Nucleotide Polymorphism Genotyping and Gene Copy Number Evaluation

 Last Update: 2020-12-13
 Source: Internet
 Author: User

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dna polymerase enzyme

dna polymerase iii

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The Invader� assay (Third Wave Technologies) is a homogeneous, isothermal
DNA
probe-based method for sensitive detection of nucleic acid sequences. Invader reactions are performed directly on genomic DNA or total RNA targets; however, polymerase chain reaction- or reverse transcriptase polymerase chain reaction-amplified products can also be used. Detection is achieved through target-specific signal amplification instead of target amplification. The assay is a highly accurate and specific detection method for both qualitative and quantitative analysis of single-nucleotide changes, insertions or deletions, gene copy number, infectious agents, and gene expression.

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