echemi logo
Product
  • Product
  • Supplier
  • Inquiry
    Home > Biochemistry News > Biotechnology News > IPG-Dalt of Very Alkaline Proteins

    IPG-Dalt of Very Alkaline Proteins

    • Last Update: 2020-12-03
    • Source: Internet
    • Author: User
    Search more information of high quality chemicals, good prices and reliable suppliers, visit www.echemi.com
    Compared to classical two-dimensional electrophoresis (2-DE) with carrier ampholytes (1 ,2 ), the advent of 2-DE with immobilized pH gradients (IPG-Dalt) (3 ) has produced significant improvements in 2-D electrophoretic separation with respect to:
    1. 
    Higher resolution by using well-defined narrow-pH gradients (3 ).
    2. 
    Improved reproducibility, as was demonstrated by interlaboratory comparisons (4 ,5 ).
    3. 
    Higher loading capacity for micropreparative runs, which has accelerated spot identification by microsequencing and mass spectrometry (6 9 ).
    4. 
    The possibility to resolve—under steady-state conditions—alkaline proteins, which are normally lost by the cathodic drift of carrier ampholyte focusing or separated by nonequilibrium pH gradient electrophoresis (NEPHGE) with limited reproducibility (10 ).
    This article is an English version of an article which is originally in the Chinese language on echemi.com and is provided for information purposes only. This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed description of the concern or complaint, to service@echemi.com. A staff member will contact you within 5 working days. Once verified, infringing content will be removed immediately.

    Contact Us

    The source of this page with content of products and services is from Internet, which doesn't represent ECHEMI's opinion. If you have any queries, please write to service@echemi.com. It will be replied within 5 days.

    Moreover, if you find any instances of plagiarism from the page, please send email to service@echemi.com with relevant evidence.