Scientists at the University of Exeter in the UK have developed a new method that allows users to measure the pH of individual bacteria before, during and after antibiotic treatment
This study, published in the journal mBio, laid the foundation for understanding the special characteristics of bacteria that survive antibiotic treatment, so that new methods can be developed for them
The research team at the University of Exeter found that even before antibiotic treatment, the intracellular pH of common infections that cause E.
coli cells to survive is more acidic than cloned cells destroyed by antibiotic treatment
These surviving cells are called persistent cells because they are responsible for persistent bacterial infections and help develop antibiotic resistance
Antibiotic resistance is one of the most pressing public health challenges, threatening the ability to effectively fight infectious diseases
It is estimated that by 2050, about 10 million people will die from infections every year
A research team at the University of Exeter discovered a mechanism that allows persistent bacteria to have an acidic pH
By measuring the genetic properties of these cells, they found that the two cellular processes of tryptophan metabolism and carboxylic acid catabolism are responsible for the low pH of persistent bacteria
Stefano Pagliara, a biophysicist at the University of Exeter's Institute of Life Systems, who led the study, said: "Our findings indicate that the control of intracellular pH represents a strategy for bacteria to survive antibiotic treatment
We The new data from ”suggests a strategy for the development of antibiotics that can interfere with key cellular components of persistent substances and reduce their acidity
The team is currently working to expand this research to find out whether cell acidity plays a key role in the antibiotic resistance of other key bacterial pathogens such as Pseudomonas aeruginosa and Bokholderia pseudo-Omar, and Identify drug molecules that can change the pH of persistent cells before antibiotic treatment
Journal Reference :
Olivia Goode, Ashley Smith, Ashraf Zarkan, Jehangir Cama, Brandon M.
Invergo, Daaniyah Belgami, Santiago Caño-Muñiz, Jeremy Metz, Paul O’Neill, Aaron Jeffries, Isobel H.
Norville, Jonathan David, David Summers , Stefano Pagliara.
Persister Escherichia coli Cells Have a Lower Intracellular pH than Susceptible Cells but Maintain Their pH in Response to Antibiotic Treatment .
MBio , 2021; DOI: 10.