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Isolation and Analysis of Small RNAs from Virus-Infected Plants

 Last Update: 2021-01-18
 Source: Internet
 Author: User

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In this chapter, we detail some of the methods available to the researcher for isolating and analyzing virus-derived small RNAs (vsRNAs). These methods have been successfully used for four plant viruses:
Cucumber mosaic virus
(CMV), including the CMV Y-Satellite,
Turnip mosaic virus
(TuMV),
Potato leaf roll virus
(PLRV), and
Tomato spotted wilt virus
(TSWV) from inoculated
Arabidopsis thaliana
plants (Fusaro et al. EMBO Rep 7:1168–1175, 2006; Curtin et al. FEBS Lett 582:2753–2760, 2008). The protocols presented here can also be employed for the isolation of non-virus related small RNAs such as microRNAs (miRNAs) and hairpin RNA (hpRNA).

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