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    Home > Biochemistry News > Plant Extracts News > Isolation and Analysis of Small RNAs from Virus-Infected Plants

    Isolation and Analysis of Small RNAs from Virus-Infected Plants

    • Last Update: 2021-01-18
    • Source: Internet
    • Author: User
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    In this chapter, we detail some of the methods available to the researcher for isolating and analyzing virus-derived small RNAs (vsRNAs). These methods have been successfully used for four plant viruses:
    Cucumber mosaic virus
    (CMV), including the CMV Y-Satellite,
    Turnip mosaic virus
    Potato leaf roll virus
    (PLRV), and
    Tomato spotted wilt virus
    (TSWV) from inoculated
    Arabidopsis thaliana
    plants (Fusaro et al. EMBO Rep 7:1168–1175, 2006; Curtin et al. FEBS Lett 582:2753–2760, 2008). The protocols presented here can also be employed for the isolation of non-virus related small RNAs such as microRNAs (miRNAs) and hairpin RNA (hpRNA).
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