Isolation of Natural Products by Low-Pressure Column Chromatography
-
Last Update: 2020-12-15
-
Source: Internet
-
Author: User
Search more information of high quality chemicals, good prices and reliable suppliers, visit
www.echemi.com
This chapter deals with the isolation of natural products using low-pressure liquid column chromatography (LPLC). A brief summary of the adsorption and size-exclusion processes involved in the LPLC is presented. Different types of stationary phases used in both adsorption and size-exclusion LPLC are described along with examples of each type used in LPLC. Operational parameters are also discussed in detail in relation to column packing (both wet and dry), column equilibration, sample application, and column development. An outline of generic procedures for adsorption and size-exclusion LPLC is provided. Sixty-one specific examples of the use of LPLC for the isolation of various natural products, including flavones, coumarins, alkaloids, and triterpenoid saponins from various plants are given. Many of these examples incorporate a combination of adsorption and size-exclusion chromatography to obtain specific compounds.
This article is an English version of an article which is originally in the Chinese language on echemi.com and is provided for information purposes only.
This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of
the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed
description of the concern or complaint, to
service@echemi.com. A staff member will contact you within 5 working days. Once verified, infringing content
will be removed immediately.