Isolation of Plant RNA
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Last Update: 2021-03-06
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Source: Internet
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Author: User
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Efficient extraction of high quality RNA from a variety of plant tissues is an important first step in many procedures, such as analysis of gene expression, c
DNA
library construction, and in vitro translation. This procedure, which is essentially as described in Draper et al. (
1
), involves grinding and phenol extraction of plant material followed by differential precipitation of RNA with sodium acetate. The protocol has been successful with leaf material and cultured cells from a large number of species, however, slight adjustments may be necessary to optimize extraction from other tissues.
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