It is revealed that long-chain non-coding RNAs express dynamic changes and effects during primate brain development and aging.

Recently, Li Jiali, Hu Xintian and Zheng Yongtang of Kunming Institute of Zoology of the Chinese Academy of Sciences, in cooperation with Wang Xiangxuan of the School of Life Sciences of the University of Science and Technology of China and Wuhan Life Beauty Biotechnology Company, revealed the dynamic changes and functions of long-chain non-coded RNAs in the development and aging of primate brains.
the results of the study, published online July 7 in Genome Research, are based on the annotation and cluster analysis of spatiotemporal-and sex-related lncRNA expression in Rhesus macaque brain.
brain development and aging process is very complex, involving genetic, age and environmental factors such as regulation and influence, including age and environment and other major factors caused by the changes in embedergene regulation is considered to be one of the important links.
90 percent of DNA in the genome has transcriptional activity, and only 1.5 percent of these genes function as encoded proteins, while most do not, including long-chain non-coding RNAs (lncRNAs).
recent years, more and more research evidence reveals that the egenesitical regulation of lncRNAs plays an important role in the occurrence and development of many life processes and diseases in the body.
studies show that the regulatory effect of lncRNAs mediated is mainly achieved by changing chromosomal composition and affecting the stability and translation of mRNAs by combining as architectural molecules with target molecules.
Although studies have found that lncRNAs show very rich expressions and changes in neurons in the mammalian brain, knowledge of their function in neurons, especially during brain development and aging in primates, remains unknown.
to this end, Li Jiali and his collaborators successfully analyzed the dynamic change characteristics of the brain development and aging process of Chinese macaques through RNA-seq and CAGE-seq deep sequencing and analysis, combined with a series of experimental methods and means such as in-place hybridization and functional verification of the original neuron system.
study found that brain-specific lncRNAs showed high regional, gender, and age expression differences, and based on these differences, a total of 18 different lncRNAs and 14 different mRNAs expression modules were found.
the expression change characteristics of mRNAs, the expression characteristics of macaque brain-specific lncRNAs showed higher dynamic change characteristics during development and aging.
, using CAGE-seq to sequence and analyze the lncRNA and mRNA initiator regions, it was found that the starter region changes of lncRNAs showed more significant gender differences and space-time variation characteristics than mRNAs.
Further studies have found that there are multiple positive and negative regulatory relationships between lncRNAs and mRNAs, in which negative regulatory relationships between lncRNAs and mRNAs for specific expression in the cerebral cortic regions play an important regulatory role in the structural and neuron function changes of the cerebral cortation during development and aging. The results of
and above provide a new experimental basis for further understanding and understanding the complex brain structure and advanced cognitive function of primates, as well as a new research direction for further exploring the pathogenesis of neurological diseases related to brain development and aging and new targets for treatment.
The co-authors of this article are Liu Shiling, Ph.D. student of Kunming Animal Institute, Assistant Researcher Wang Zhengbo and Tian Renrong, Dr. Chen Dong of Wuhan Life Beauty Biology Company, Ph.D. student Zhang Bowen of the School of Life Sciences of China University of Science and Technology, and the communication author is Li Jiali, Researcher of Kunming Animal Institute, Wang Xiangxuan, Professor of China University of Science and Technology, and Dr. Zhang Yi of Wuhan Life Aesthetics Company.
project is supported by the 100-person program of the Chinese Academy of Sciences, the "973" project of the Ministry of Science and Technology, the National Natural Science Foundation of China and the Special Fund of the Chinese Academy of Sciences Class B Pilot.
research center of Kunming Animal Research Institute has provided important resources and technical support for the research.
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