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Monoallelic gene expression refers to the phenomenon in which all transcripts of a gene in the cells of a double-body organism come from an allele.
analysis of cell expression spectrometry (bulk analysis) at the population level shows that the mutual inhibition between imprinting effect and allogens is two possible mechanisms for producing single allogen expression.
because population-level analysis may underestimate the universality of single allogen expression within a single cell, the study of single-cell level can reveal the phenomenon of single allogen expression in cells in a more comprehensive way
, the Institute of Genetics and Developmental Biology of the Chinese Academy of Sciences, a number of research groups to rice leaf meat cells as the object, cooperative research and revealed the existence of a wide range of single-isotropic gene expression in plants. The
Coking Rainbell Study Group applied single-cell transcription group assays (single-cell RNA-seq) to plant cells and obtained a single-cell expression spectrum of leaf-meat cells from two rice subsectives (93-11 and Nipponbare) and their orthopaedic offspring (F1).
Qian Wenfeng and Wang Xiujie used mononucleotide polymorphisms between the 93-11 and Nipponbare subseeds to estimate the expression of two allergens in F1 cells.
to define the gene expressed by single allergens in cells by comparing the expression of allergens.
studies have shown that about two-thirds of genes in a single cell are expressed by single allergens.
further studies have found that the independence and randomness of allergene expression can explain the phenomenon of single allergen expression in cells.
the study revealed the universality and possible mechanism of single-isogenic gene expression at the single-cell level, which laid the foundation for further study of the regulation of gene expression in cells.
research is published online at Science Bulletin.
research was funded by the Ministry of Science and Technology's 973 project, the "People's Program" of the Central Group Department and the National Key Laboratory of Plant Genomics.
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