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Due to the existence of complications such as hyperglycemia, insulin resistance and diabetes (T2DM), obesity has become a major global health burden
Long-term consumption of a high-fat diet (HF) not only causes obesity and insulin resistance, but also causes histopathological changes in the pancreas
Magnetic resonance imaging (MRI) acquisition: At the end of the 16th week, the rats were anesthetized by intraperitoneal injection of 40 mg/kg pentobarbital (single dose) and placed in a prone position on a 1.
Determination of lipid peroxidation in pancreatic tissue: Simply put, pancreatic tissue is sliced and immersed in CelLytic™ MT cell lysis reagent (Sigma-Aldrich), which contains 1% complete protease inhibitor cocktail (Roche Applied Science)
Western blot analysis: prepare lysate homogenate of pancreas or kidney tissue
Rats fed a high-fat diet showed metabolic abnormalities, including increased body weight, visceral fat body weight, plasma insulin, plasma cholesterol, homeostasis model assessment (HOMA) index, and TAUCg, indicating the presence of obese insulin resistance and glucose intolerance
Improve pancreatic oxidative stress, endoplasmic reticulum stress, inflammation and apoptosis in obese rats, and restore autophagy in the kidneys
Figure 1 Research protocol (a).
Figure 1 Research protocol (a).
Effects of Glipizide and Vidagliptin on the metabolic parameters of obese rats induced by high-fat diet
Effects of Glipizide and Vidagliptin on the metabolic parameters of obese rats induced by high-fat dietFigure 2 The effect of dapagliflozin and vedagliptin treatment on pancreatic MDA content (A), 4-HNE (B) and SOD2 expression (C) in obese rats
Figure 2 The effect of dapagliflozin and vedagliptin treatment on pancreatic MDA content (A), 4-HNE (B) and SOD2 expression (C) in obese rats
Figure 3 Treatment of dapagliflozin and vedagliptin on p62(A), LC3B(B), Beclin-1(C), Atg5(D), kidney H&E staining(E), kidney semi-quantitative treatment in obese rats The effects of damage score (F), PAS staining (G) and glomerulosclerosis index (H)
Figure 3 Treatment of dapagliflozin and vedagliptin on p62(A), LC3B(B), Beclin-1(C), Atg5(D), kidney H&E staining(E), kidney semi-quantitative treatment in obese rats The effects of damage score (F), PAS staining (G) and glomerulosclerosis index (H)
In summary, this study confirmed that dapagliflozin can alleviate pancreatic injury, pancreatic oxidative stress, endoplasmic reticulum stress, inflammation, and apoptosis in obese rats, and play a renal protective effect by restoring autophagy signaling pathways
Jaikumkao K, Promsan S, Thongnak L,et al.
Dapagliflozin ameliorates pancreatic injury and activates kidney autophagy by modulating the AMPK/mTOR signaling pathway in obese rats.
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