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    Home > Active Ingredient News > Endocrine System > J Cell Physiol: Dapagliflozin improves pancreatic injury in obese rats and activates renal autophagy by modulating AMPK/mTOR signaling pathway

    J Cell Physiol: Dapagliflozin improves pancreatic injury in obese rats and activates renal autophagy by modulating AMPK/mTOR signaling pathway

    • Last Update: 2021-11-12
    • Source: Internet
    • Author: User
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    Due to the existence of complications such as hyperglycemia, insulin resistance and diabetes (T2DM), obesity has become a major global health burden


    Long-term consumption of a high-fat diet (HF) not only causes obesity and insulin resistance, but also causes histopathological changes in the pancreas


    Magnetic resonance imaging (MRI) acquisition: At the end of the 16th week, the rats were anesthetized by intraperitoneal injection of 40 mg/kg pentobarbital (single dose) and placed in a prone position on a 1.


    Determination of lipid peroxidation in pancreatic tissue: Simply put, pancreatic tissue is sliced ​​and immersed in CelLytic™ MT cell lysis reagent (Sigma-Aldrich), which contains 1% complete protease inhibitor cocktail (Roche Applied Science)


    Western blot analysis: prepare lysate homogenate of pancreas or kidney tissue


    Rats fed a high-fat diet showed metabolic abnormalities, including increased body weight, visceral fat body weight, plasma insulin, plasma cholesterol, homeostasis model assessment (HOMA) index, and TAUCg, indicating the presence of obese insulin resistance and glucose intolerance


    Improve pancreatic oxidative stress, endoplasmic reticulum stress, inflammation and apoptosis in obese rats, and restore autophagy in the kidneys


    Figure 1 Research protocol (a).


    Figure 1 Research protocol (a).


    Effects of Glipizide and Vidagliptin on the metabolic parameters of obese rats induced by high-fat diet

    Effects of Glipizide and Vidagliptin on the metabolic parameters of obese rats induced by high-fat diet

    Figure 2 The effect of dapagliflozin and vedagliptin treatment on pancreatic MDA content (A), 4-HNE (B) and SOD2 expression (C) in obese rats


    Figure 2 The effect of dapagliflozin and vedagliptin treatment on pancreatic MDA content (A), 4-HNE (B) and SOD2 expression (C) in obese rats


    Figure 3 Treatment of dapagliflozin and vedagliptin on p62(A), LC3B(B), Beclin-1(C), Atg5(D), kidney H&E staining(E), kidney semi-quantitative treatment in obese rats The effects of damage score (F), PAS staining (G) and glomerulosclerosis index (H)


    Figure 3 Treatment of dapagliflozin and vedagliptin on p62(A), LC3B(B), Beclin-1(C), Atg5(D), kidney H&E staining(E), kidney semi-quantitative treatment in obese rats The effects of damage score (F), PAS staining (G) and glomerulosclerosis index (H)


    In summary, this study confirmed that dapagliflozin can alleviate pancreatic injury, pancreatic oxidative stress, endoplasmic reticulum stress, inflammation, and apoptosis in obese rats, and play a renal protective effect by restoring autophagy signaling pathways


    Jaikumkao K, Promsan S, Thongnak L,et al.


    Dapagliflozin ameliorates pancreatic injury and activates kidney autophagy by modulating the AMPK/mTOR signaling pathway in obese rats.


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