J Ethnopharmacol: Hefei Research Institute has made progress in the pharmacology of Reishi's anti-cancer.

Recently, Huang Qing, a researcher at the Ion Beam Bioengineering and Green Agriculture Research Center of the Institute of Intelligent Machinery of hefei Institute of Material Sciences of the Chinese Academy of Sciences, made new progress in the pharmacological research of Reishi anti-cancer, and found that Reishi acid D can induce apoptosis and autophagy cell death of esophageal scale cancer cells at the same time.
study was published in the journal Pharmacological Research, Journal of Ethnopharmacology.
as a traditional Chinese medicine has more than two thousand years of medicinal history, and has a variety of medicinal value.
, Reishi acid is an important pharmacological component with anti-tumor and inhibition of cell proliferation in Reishi, but its specific mechanism of action still has many problems to be studied and clarified.
years, Huang Qing task force has been carrying out research on reishi breeding and functional development.
The study focused on the mechanism of reishic acid's effect on cancer cells, and the results showed that reishic acid significantly reduced the cell activity of esophageal scale cancer cells at different times and doses, and significantly blocked cell cycles during the G2/M phase as the dose increased.
can not only induce apoptosis, but also induce the occurrence of autophagy.
by detecting autophagy marker proteins (LC3, P62, Beclin-1) to show that reishic acid can increase the expression of autophagy.
further detection of autophagy by autophagy double fluorescence marker (GFP-RFP-LC3) method, it was found that reishi acid can block autophagy flow, by blocking the fusion of autophagy and lysosomes, resulting in autophagy cell death.
further analysis found that reishi acid reduced the expression of lysosome membrane protein (LAMP-2), the use of Mito-tracker fluorescent probe marker lysosome found reishic acid neutral lysosome acidity, resulting in lysosomes can not be fused with autophagy small bodies, resulting in autophagy blocking leading to cell death.
also found that the combination of reishic acid and chloroquine can significantly increase the proportion of apoptosis.
by analyzing expression changes such as cell vitality, caspase, LC3, etc. by adding an inhibitor of caspase protein (Z-VAD-FMK), it is further verified that reishic acid can induce autophagy cell death.
At the molecular level, Reishi acid by mediating the PI3K/AKT/mTOR path, reducing the level of AKT protein phosphorylation triggers apoptosis of the caspase path, while reducing the level of mTOR protein phosphorylation induces autophagy, resulting in autophagy blocking, resulting in autophagy cell death.
this study provides theoretical basis and support for the treatment of reishic acid as an anti-tumor adage for esophageal squamous cancer.
the research work is supported by the National Natural Science Foundation of China and horizontal topics.
a: A diagram of reishi acid-induced apoptosis and autophagy death of esophageal scaly cancer cells b: A diagram of autophagy cell death caused by reishic acid blocking autophagy flow.