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Background: Graves' disease (GD) is an autoimmune disease, accounting for about 0.
Graves' disease (GD) is an autoimmune disease, accounting for about 0.
Results: The lowest concentration of TRAb detected by dot blot was 100 ng, and the dilution of antiserum was 1:60
Figure 1 FIGURE 1 cloning of HTSHR-169 in pET28a(+) vector
Figure 1 FIGURE 1 cloning of HTSHR-169 in pET28a(+) vector
Figure 2 (A) The expression profile of hTSHR-169 fusion protein induced by 0.
Figure 2 (A) The expression profile of hTSHR-169 fusion protein induced by 0.
Figure 3 (A) Immunoreactivity of 20 known GD and control samples
Figure 3 (A) Immunoreactivity of 20 known GD and control samples
Table 1 Comparison of detection of TRAb by ELISA and dot blot
Table 1 Comparison of detection of TRAb by ELISA and dot blotConclusion: Although the detection effect of dot blot method is lower than that of ELISA method, dot blot method is a simple and rapid diagnostic method, which is suitable for the diagnosis of GD in rural areas that are sometimes difficult for medical institutions to access
Although the detection effect of dot blot method is lower than that of ELISA method, dot blot method is a simple and rapid diagnostic method suitable for the diagnosis of GD in rural areas that are sometimes difficult for medical institutions to access
Comparative performance of ELISA and dot blot assay for TSH-receptor antibody detection in Graves' disease.
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