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    Home > Active Ingredient News > Endocrine System > JCLA: Comparison of ELISA and Dot Blot for the Detection of Graves' Disease TSH Receptor Antibodies

    JCLA: Comparison of ELISA and Dot Blot for the Detection of Graves' Disease TSH Receptor Antibodies

    • Last Update: 2022-04-16
    • Source: Internet
    • Author: User
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    Background: Graves' disease (GD) is an autoimmune disease, accounting for about 0.


    Graves' disease (GD) is an autoimmune disease, accounting for about 0.


    Results: The lowest concentration of TRAb detected by dot blot was 100 ng, and the dilution of antiserum was 1:60


    Figure 1 FIGURE 1 cloning of HTSHR-169 in pET28a(+) vector


    Figure 1 FIGURE 1 cloning of HTSHR-169 in pET28a(+) vector


    Figure 2 (A) The expression profile of hTSHR-169 fusion protein induced by 0.


    Figure 2 (A) The expression profile of hTSHR-169 fusion protein induced by 0.


    Figure 3 (A) Immunoreactivity of 20 known GD and control samples


    Figure 3 (A) Immunoreactivity of 20 known GD and control samples


    Table 1 Comparison of detection of TRAb by ELISA and dot blot

    Table 1 Comparison of detection of TRAb by ELISA and dot blot

    Conclusion: Although the detection effect of dot blot method is lower than that of ELISA method, dot blot method is a simple and rapid diagnostic method, which is suitable for the diagnosis of GD in rural areas that are sometimes difficult for medical institutions to access


    Although the detection effect of dot blot method is lower than that of ELISA method, dot blot method is a simple and rapid diagnostic method suitable for the diagnosis of GD in rural areas that are sometimes difficult for medical institutions to access


    Comparative performance of ELISA and dot blot assay for TSH-receptor antibody detection in Graves' disease.


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