JE: Study on the mechanism of Fuzheng Xiaozheng Recipe in alleviating liver cancer in rats

Fuzhengxiaozhen disease square by the Ming Dynasty "plague of" famous decoction recorded three scattered derived from traditional Chinese medicine, after years of clinical application, Fuzhengxiaozhen disease parties that have struck in cirrhosis and hepatocellular carcinoma (HCC) therapy Satisfactory results
.

However, its underlying mechanism is still largely unknown

Fuzheng Xiaozheng Recipe Fuzheng Xiaozheng Recipe "On Epidemic Disease " "On Epidemic Disease " Sanjia San Sanjia San

A study recently published in the Journal of Ethnopharmacology, the use of diethylnitrosamine (DEN) to induce hepatocellular carcinoma in rats, and treatment with Fuzheng Xiaozheng Decoction aims to systematically evaluate the effects of Fuzheng Xiaozheng Decoction on rats The intervention effect of HCC, and in-depth elucidation of its regulatory mechanism
.

The study used high performance liquid chromatography (HPLC) to analyze the ingredients of the prepared medicine
.

Blood biochemistry (ALT, AST, ALP, GGT, DBIL, TBIL), liver histopathology, and immunohistochemistry (Ki67) were used to evaluate the intervention effect

High-performance liquid chromatography (HPLC) analysis of the prepared pharmaceutical ingredients High-performance liquid chromatography (HPLC) analysis of the prepared pharmaceutical ingredients miRNA-Seq and mRNA-Seq miRNA-Seq and mRNA-Seq regulation mechanism regulation mechanism

The study identified 9 potential ingredients from Fuzheng Xiaozheng Recipe , and its anti-cancer predictions showed that almost all ingredients showed anti-cancer effects
.

9 potential ingredients 9 potential ingredients

H&E staining showed that the model group had obvious inflammation infiltration, the volume of hepatocytes was reduced , and the liver pathology of the treatment group was significantly improved
.

In addition, Ki67 immunohistochemistry also showed that the proliferation and differentiation of liver cancer cells in the treatment group was lower than that in the model group, and the proportion of Ki67+ cells in the treatment group was significantly lower than that in the model group

The model group has obvious inflammation infiltration, and the hepatocyte volume is reduced.

PPAR signaling pathway, arachidonic acid metabolism, bile secretion PPAR signaling pathway, arachidonic acid metabolism, bile secretion

The QRT-PCR results showed that among the 4 DEmiRs, 3 expressed consistent with miRNA-Seq, and the 6 selected mRNAs all showed the same expression trend as mRNA-Seq
.

Both qRT-PCR and RNA-Seq showed that the expression trend of this gene was dose-dependent, which was consistent with the above results

In order to further confirm the regulating effect of Fuzheng Xiaozheng Recipe on lipid-related metabolism and inflammation, the study detected the contents of 4 lipid-related indexes (TC, TG, LDL-C, HDL-C) and 4 kinds of cells in the serum.
The expression level of factors (IL-2, IL-6, IL-10, IFN-γ)
.

After the intervention of Fuzheng Xiaozheng Recipe, TC and TG showed a downward trend from the model group to the high-dose treatment group, and the contents of LDL-C and HDL-C showed an upward trend

(TC, TG, LDL-C, HDL-C) (TC, TG, LDL-C, HDL-C) (IL-2, IL-6, IL-10, IFN-γ) (IL-2, IL- 6.

Regulate lipid-related metabolism and enhance anti-inflammatory ability Regulate lipid-related metabolism and enhance anti-inflammatory ability

 

Literature source:

Literature source:

Li Xia.

Li Xia.
, Yu Han.
, Gong Yanju.
, Wu Peijie.
, Feng Quan-Sheng.
, Liu Chao.
(2021).
Fuzheng Xiaozheng prescription relieves rat hepatocellular carcinoma through improving anti-inflammation capacity and regulating lipid related metabolisms.
J Ethnopharmacol , undefined(undefined), 114801.
doi:10.
1016/j.
jep.
2021.
114801 leave a message here