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Original title: Key genes for controlling starch quality have been found
many foods rich in
starch
are popular, such as potatoes. But excessive starch intake can easily lead to obesity and diabetes. One type of starch is the exception, that is, resistant starch, such very "personality" starch is not easy to be digested and absorbed by the human body, in the intestines by microorganisms broken down to produce beneficial short-chain fatty acids. Therefore, it is important to improve the content of resistant starch in starch.
recently, researchers at the University of Copenhagen in Denmark successfully increased the content of straight-chain starch and resistant starch in
potato
starch through the
national non-GMO
method, and made a preliminary analysis of its rationale. The findings were published in the prestigious international journal Plant Biotechnology Journal.
have shown that the total starch of potatoes contains about 20 to 30% of straight-chain starch and 70-80% of branch-chain starch, in which the content of straight-chain starch is positively related to the content of resistant starch. The researchers used a wild material with high straight-chain starch content (recessive characteristics) as a supply, interbreeded with cultivated potatoes and multi-generational recission, and obtained a number of copies with cultivated potatoes on the ground part of no significant difference, but the proportion of straight-chain starch and resistant starch in tuber starch significantly increased.
The researchers speculated that the recessive esoteric physicophysics in the recission material may have been caused by the dose effect of
gene
in the four-fold potato cultivation species, so they selected 15 recirective materials with high straight-chain starch content for genome mixing pool sequencing. Through the SNP hesitation analysis, three regions with significantly lower heptosysty were found as candidate intervals for controlling the direct chain starch content. In one of the zones, the researchers found two genes associated with starch synthesis.
combined with transcriptional group analysis, the researchers found that the expression of iso-amylase genes in high-straight-chain starch content of the return material was reduced by 556 times. The gel electrophoresis and activity analysis of iso-amylase showed that the recissive material of high-straight-chain starch was missing from the regeneration parent. In conjunction with other related experiments, the researchers speculated that the absence of iso-amylase in potatoes may be the main cause of high straight-chain starch content, but other genes also played a role in the process.
This time, through the joint analysis of genome mixing pool sequencing and transcription group, the researchers initially identified the key genes to control starch quality, which provided new genetic resources for potato quality improvement, and also provided theoretical guidance for the study of the formation mechanism of straight-chain starch and resistant starch.
about the paper:
.