Kochia saponins lc

Kochia saponins lc

Kochioside Ic

【Structural formula】

[Molecular formula and molecular weight] C ₄₁ H ₆₄ O ₁₃ ; 764.

[Source] The dried and mature fruit of Kochia scoparia (L.

【Properties】White powder

This product is soluble in methanol

Melting point: 220～221℃

【Purity Check】

TLC

1.

Developing solvent of chloroform - methanol - water (16:9:2)

Check the color development of 10% sulfuric acid ethanol solution, check under sunlight

2.

Developing solvent of chloroform - ethyl acetate - methanol - water (15:40:22: 10) lower

Check the color development of 10% sulfuric acid ethanol solution, check under sunlight

High performance liquid chromatography

Column Agilent Zorbax SB C ₁₈ , 5um (4.

Mobile phase methanol-water- glacial acetic acid (85:15:0.

Evaporative light scattering detection, drift tube temperature 100℃, carrier gas flow rate 1.

Differential calorimetry

Start temperature 50℃, end temperature 250℃, heating rate 5℃/min

【Structural identification】204

3411, 2944, 1692, 1463, 1388, 1364, 1265, 1161, 1080, 1039

ESI-MS m/z: 787[M+Na] ⁺

¹ H-NMR (CD ₅ OD) δ: 0.

¹³ C-NMR (CD ₅ OD) δ: 15.

【Storage】Dry and airtight
.