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    Home > Active Ingredient News > Drugs Articles > Leukocyte count (tube method)

    Leukocyte count (tube method)

    • Last Update: 2014-08-28
    • Source: Internet
    • Author: User
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    1、 In principle, dilute the blood 20 times with dilute acetic acid to dissolve all the red blood cells, drop them into the white blood cell counting pool, count them under the microscope, and get the number of white blood cells per cubic millimeter Equipment and reagents 1 Equipment biomicroscope, Neubauer's blood cell counting pool, 0.02 ml pipette, small test tube, pricking needle, 2 ml pipette 2 Reagent leukocyte diluent: use 3% acetic acid solution 3 The diluent is prepared with 3% acetic acid solution Take 3 ml of glacial acetic acid, add distilled water to 100 ml and mix One or two drops of methylene blue or gentian violet can also be added to the above diluent for easy observation The diluent should be filtered frequently to remove the effects of dust, foreign matter or microorganisms 4 Method 1 Add 0.38 ml of diluent into the small test tube, then take 0.02 ml of blood from the earlobe or finger with hemoglobin pipette, blow it into the diluent immediately, wash the blood on the pipette wall into the diluent, and shake it gently 2 Use a small dropper to take a small amount of liquid from the above-mentioned shaken diluent and add it into the blood cell counting pool Let it stand for 1-2 minutes Count the total number of white blood cells in four large squares with a low power microscope, and then multiply the number by 50 to get the number of white blood cells per cubic millimeter.
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