Linear range and lower limit of determination of streptomycin, dihydrostreptomycin and kanamycin residues in milk powder, milk, puffer fish, eel and royal jelly

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8 Linearity range and lower limit of determination

The concentrations of streptomycin , dihydrostreptomycin and kanamycin are 5.
0μg/L, 10.
0μg/L, 20.
0μg/L, 50.
0μg/L, 100.
0μg/L and 200.
0μg/L.
Under the conditions of mass spectrometry, the sample volume was 30μL and the measurement was carried out.
The peak area of ​​the response value of the quantitative ion pair of streptomycin, dihydrostreptomycin and kanamycin was plotted with the solution concentration, and the standard curve obtained was linear.
, Meet the quantitative requirements, see Table 3-12
.

Table 3-12 Linear range, linear equation and correlation coefficient of streptomycin, dihydrostreptomycin and kanamycin

When the milk sample of streptomycin, dihydrostreptomycin and kanamycin is added at 10.
0μg/kg, the milk powder sample of streptomycin, dihydrostreptomycin and kanamycin is added at 80.
0ug/kg, its The signal-to-noise ratio is greater than 10, and good recovery and precision are obtained.
From this, it is determined that the detection limits of streptomycin, dihydrostreptomycin and kanamycin in milk are all 10.
0ug/kg.
The detection limits of dihydrostreptomycin, dihydrostreptomycin and kanamycin were all 80.
0μg/kg
.