Liquid paraffin method of microbial species preservation method.

Biotechnology Channel News: Liquid paraffin method is also known as mineral oil preservation method, regular transplant preservation method auxiliary method.
refers to the bacteria inoculated on the appropriate sloped culture base, under the most suitable conditions to grow the bacteria grow strong bacteria after injection into the sterilized liquid paraffin, so that it covers the entire slope, and then placed upright in the low temperature (4 to 6 degrees C) dry place for preservation of a species preservation method.
operating steps are as follows: 1, liquid paraffin preparation selection of high-quality chemical pure liquid paraffin, liquid paraffin is packed and stuffed, wrapped in psoriate paper, using the following two ways to sterilize: 121 degrees C wet heat sterilization 30min, 40 degrees C thermostat evaporation of water, after sterile inspection.
sterilized for 2 hours at 160 degrees C, cooled and spared after sterile examination.
2, bevel culture preparation reference to the regular transplant method.
3, infusion paraffin will be sterile liquid paraffin in sterile conditions injected into the cultured fresh slope culture, the liquid surface is about 1cm above the top of the slope, so that the bacteria and air isolation.
4, preservation 4.1 injection of liquid paraffin species slope in an upright state at low temperature (4 to 6 degrees C) dry place preservation, preservation time of 2 to 10 years.
4.2 should be checked regularly during storage, if the culture base exposed liquid surface, should be timely replenishment of sterile liquid paraffin.
5, the resumption of culture recovery culture, pick a small number of bacteria transferred to the appropriate fresh media, growth and reproduction, and then re-transfer once.
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