List of items used in molecular biology experiments.
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Last Update: 2020-10-30
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Source: Internet
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Author: User
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the following items in each group (two people): . Gun head box 1000ul 1 / group gun head box 200ul 1 / groupsmall
beaver
2 / group triangle bottle 2 / groupculture
dishes 2 / group double panel 1 / groupmarker pen 2 1 / group
liquidator
1000ul 1 only / groupliquidator 200ul 1 / group liquidator 20ul 1 / groupitems below 2 groups (four people) set:shifter 0. 2ul 1 x /2 set gun rack 1 x /2 groupresearch 1 x /2 group label paper 1 sheet /2 groupgun head box 10ul 1 x /2 set tweezers 1 x /2 setscissors 1 x 2 groups
quantum
(10ml) 1 x /2barrel (50ml) 1 x 2 groupsCheck correctly and ask each group owner to sign the list.experimental content: 1, the correct use of trace fluid extractorrange of the correct choice (do not take the exponential excessive range) swee lightly put down the pusher to the first vertical sampling, when the sample down pusher to the second gear.1, put the gun head in the gun head box, sterilization.3, put 0. 2 ml, 1. 5 ml centrifugal tube in a small oh a berries, sterilized.4, preparation phenol - chloroform (Tris saturation phenol: chloroform: 1:1) 50 ml.5,
centrifuges are
used correctly.6, the correct use of sterilization pot.use
PCR
7 and PCR meters.8, the use of ultraviolet spectrophotometers 9, the use of gel imager 10, electrophoresis Cao and electrophoresis instrument use 11, each triangular bottle with 20 ml of LB
medium
(protein1%,
yeast
powder 0.5%, NaCl1%), sealing bacteria. Remove 2 ml of the culture base from each group before inoculation and load 1. 5ml centrifugal tube, spare.12, one bottle of GST primers per two groups (20ul100 mg/ml of ampicillin in the medium);
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